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http://dx.doi.org/10.18419/opus-6235
Autor(en): | Osipov, A. P. Gapanova, N. K. Egorov, A. M. Berezin, I. V. |
Titel: | Verfahren zur Bestimmung der alkalischen Phosphatase-Aktivität |
Erscheinungsdatum: | 1991 |
Dokumentart: | Zeitschriftenartikel |
Erschienen in: | Patent SU 1449588, 7.1.1989 (klass C 12 Q 1/32, G 01 N 33/535) |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-37497 http://elib.uni-stuttgart.de/handle/11682/6252 http://dx.doi.org/10.18419/opus-6235 |
Zusammenfassung: | The activity of alk. phosphatase is detd. by incubation of a sample with NADP (I) in a buffer medium with subsequent spectrophotometric recording of the hydrolysis product in an enzymic regeneration system of a cofactor-contg. alk. dehydrogenase and two substrates, one of which is EtOH. The sensitivity of the detn. is increased and a rapid and simplified detn. is ensured by incubation of the sample and recording of the hydrolysis product catalyzed by alk. phosphatase in a pH 8,5 - 9,1 tris-HCl buffer; By using I in reduced form at 10-7 - 10-4 M; by using horse liver alk. dehydrogenase at 5 x 10-7 - 10-5 M the cofactor regeneration system; by using p-nitroso-N,N'-dimethylamine at 5 x 10-6 - 3 x 10-4 M as th 2nd substrate; and by adding salicyclic acid amide to 2 x 10-4 - 5 x 10-7 M and Na orthophosphate to 0,05 M to the system. |
Enthalten in den Sammlungen: | 13 Zentrale Universitätseinrichtungen |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Ue_379_Osipov.pdf | 5,77 MB | Adobe PDF | Öffnen/Anzeigen |
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