Besonderheiten der Transformation und Verwertung von 4-Fluorbenzoesäure durch Corynebacterium fascians

Abstract

Corynebacterium fascians INMI KIS-1 cannot utilize 4-fluorbenzoic acid since one of the enzyme activities is absent at the terminal steps of 4-FBA preparatory metabolism. Upt to 95% of the fluoride is split off in the course of 4-FBA transformation. The quantity of 4-carboxymethylene butenolide and maleylacetate accumulated in the medium in the process of 4-FBA transformation by C. fascians INMI KIS-1 corresponds, in stochiometrical terms, to the amount of added 4-FBA. This value is far lower (26%) in a C. fascians INMI KIS-9 mutual capable of 4-FBA utilization. Therefore, C. fascians adaptation to 4-FBA should be attributed to the fact that one of the enzyme activities at the terminal steps of 4-FBA preparatory metabolism (maleylacetate reductase) either appears or noticeably rises.