Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-7769
Authors: Rajewsky, Manfred F.
Fabricius, Erika
Hülser, Dieter F.
Title: Synchronisation in vivo: temporary inhibition of DNA synthesis in the rat embryo with hydroxyurea
Issue Date: 1971
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.source: Experimental cell research 66 (1971), S. 489-492. URL http://dx.doi.org./10.1016/0014-4827(71)90706-3
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-67800
http://elib.uni-stuttgart.de/handle/11682/7786
http://dx.doi.org/10.18419/opus-7769
Abstract: As part of experimental studies on the synchronisation of mammalian cell populations in vivo, the proliferative response of rat embryo cells (18th day of gestation) was investigated following transplacental administration of hydroxyurea (HU). DNA synthesis was strongly inhibited by i.p. injection of a single dose of 0.25 or 0.5 mg HU/g body weight, for periods of 2.5 and 4.5 h, respectively. Reversal of blocks occurred rapidly due to the short half-life (45 min) of the inhibitor in the embryo. The resulting synchronizing effects were analysed in terms of mitotic indices and rate of 3H-TdR incorporation for a period of 18 h after administration of HU.
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