Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-8023
Authors: Rubio, Miguel Angel
Engesser, Karl-Heinrich
Knackmuss, Hans-Joachim
Title: Microbial metabolism of chlorosalicylates: effect of prolonged subcultivation on constructed strains
Issue Date: 1986
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.source: Archives of microbiology 145 (1986), S. 123-125. URL http://dx.doi.org./10.1007/BF00446768
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76569
http://elib.uni-stuttgart.de/handle/11682/8040
http://dx.doi.org/10.18419/opus-8023
Abstract: The hybrid strain Pseudomonas sp. WR4016 was subcultivated with increasing concentrations of 5-chlorosalicylate (5rarr10 mM) as sole carbon source over a period of 9 months. At intervals of approximately 3 months derivative strains WR4017, WR4018 and WR4019 were isolated which exhibited higher growth rates and increased substrate tolerance. Comparative analysis of the turnover rates of the key enzymes in chlorosalicylate degradation showed that the adaptation process did not result from structural modifications of these proteins. Instead, balanced over-production of the salicylate hydroxylase and catechol 1,2-dioxygenase prevented the accumulation of toxic chlorocatechols and accounted for the reduction of the doubling times with 4- or 5-chlorosalicylate. A comparative analysis of a genetically engineered chlorosalicylate degrader PL300-1 showed similar regulatory patterns as the most advanced isolate WR4019 from the adaptation series.
Appears in Collections:15 Fakultätsübergreifend / Sonstige Einrichtung

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