Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-8023
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dc.contributor.authorRubio, Miguel Angelde
dc.contributor.authorEngesser, Karl-Heinrichde
dc.contributor.authorKnackmuss, Hans-Joachimde
dc.date.accessioned2012-08-22de
dc.date.accessioned2016-03-31T11:44:57Z-
dc.date.available2012-08-22de
dc.date.available2016-03-31T11:44:57Z-
dc.date.issued1986de
dc.identifier.other371416175de
dc.identifier.urihttp://nbn-resolving.de/urn:nbn:de:bsz:93-opus-76569de
dc.identifier.urihttp://elib.uni-stuttgart.de/handle/11682/8040-
dc.identifier.urihttp://dx.doi.org/10.18419/opus-8023-
dc.description.abstractThe hybrid strain Pseudomonas sp. WR4016 was subcultivated with increasing concentrations of 5-chlorosalicylate (5rarr10 mM) as sole carbon source over a period of 9 months. At intervals of approximately 3 months derivative strains WR4017, WR4018 and WR4019 were isolated which exhibited higher growth rates and increased substrate tolerance. Comparative analysis of the turnover rates of the key enzymes in chlorosalicylate degradation showed that the adaptation process did not result from structural modifications of these proteins. Instead, balanced over-production of the salicylate hydroxylase and catechol 1,2-dioxygenase prevented the accumulation of toxic chlorocatechols and accounted for the reduction of the doubling times with 4- or 5-chlorosalicylate. A comparative analysis of a genetically engineered chlorosalicylate degrader PL300-1 showed similar regulatory patterns as the most advanced isolate WR4019 from the adaptation series.en
dc.language.isoende
dc.rightsinfo:eu-repo/semantics/openAccessde
dc.subject.classificationPseudomonas , Salicylate , Stoffwechselde
dc.subject.ddc570de
dc.titleMicrobial metabolism of chlorosalicylates: effect of prolonged subcultivation on constructed strainsen
dc.typearticlede
ubs.fakultaetFakultätsübergreifend / Sonstige Einrichtungde
ubs.fakultaetFakultät Energie-, Verfahrens- und Biotechnikde
ubs.institutSonstige Einrichtungde
ubs.institutInstitut für Mikrobiologiede
ubs.opusid7656de
ubs.publikation.sourceArchives of microbiology 145 (1986), S. 123-125. URL http://dx.doi.org./10.1007/BF00446768de
ubs.publikation.typZeitschriftenartikelde
Appears in Collections:15 Fakultätsübergreifend / Sonstige Einrichtung

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