04 Fakultät Energie-, Verfahrens- und Biotechnik

Permanent URI for this collectionhttps://elib.uni-stuttgart.de/handle/11682/5

Browse

Author: search.filters.author.Hülser, Dieter F.End date: 1979

Search Results

Now showing 1 - 3 of 3
  • Thumbnail Image
    ItemOpen Access
    Survival and chromosome injury of spheroid cells after irradiation
    (1979) Dertinger, Hermann; Hülser, Dieter F.; Hinz, Gudrun
    Multicell spheroids (SPH) offer unique advantages for studying modifications of single cell radiosensitivity due to the extensive intercellular communication in a three-dimensionally organized tissue. We have been able to relate the increased radio-resistance of spheroid cells as compared to monolayer (ML) cells ("Contact Resistance") to the degree of ionic coupling between the cells as measured by a micro-electrode technique.
  • Thumbnail Image
    ItemOpen Access
    Electronmicroscopical and electrophysiological investigations on polyethylene glycol induced cell fusion
    (1978) Krähling, Hermann; Schinkewitz, Ursula; Barker, Angela; Hülser, Dieter F.
    Cells of monolayer cultures are fused by high concentrations of polyethylene glycol (PEG) with a molecular weight of approximately 1500. This process is independent of extracellular ca++ions. PEG changes transiently the surface membrane and leads to fusion only after replacing it by normal medium. Before the final fusion of two cells, the onset of ionic coupling via longer lasting pseudopodial contact can be measured. Only cells that are synchronous in the secretory and pseudopodial response to PEG may fuse with each other.
  • Thumbnail Image
    ItemOpen Access
    Membrane properties of cultured cells
    (1976) Hülser, Dieter F.
    Intercellular communication is a prerequisite for intact organisms especially during development and differentiation, and occurs as regulation over long distances (hormones), as short distance interactions (synapses) or as direct cell to cell contact. The direct cell contact is enabled by specialized membrane areas, the so called gap junctions, which allow not only the passage of ions (ionic coupling) but also of larger molecules (metabolic cooperation). The identity of these three coupling phenomena was demonstrated with cell cultures, where hormonal or neuronal regulatory processes can be excluded.