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http://dx.doi.org/10.18419/opus-12498
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DC Element | Wert | Sprache |
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dc.contributor.author | Welden, Melanie | - |
dc.contributor.author | Poghossian, Arshak | - |
dc.contributor.author | Vahidpour, Farnoosh | - |
dc.contributor.author | Wendlandt, Tim | - |
dc.contributor.author | Keusgen, Michael | - |
dc.contributor.author | Wege, Christina | - |
dc.contributor.author | Schöning, Michael J. | - |
dc.date.accessioned | 2022-11-09T12:41:15Z | - |
dc.date.available | 2022-11-09T12:41:15Z | - |
dc.date.issued | 2022 | - |
dc.identifier.issn | 2079-6374 | - |
dc.identifier.other | 1823285724 | - |
dc.identifier.uri | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-125174 | de |
dc.identifier.uri | http://elib.uni-stuttgart.de/handle/11682/12517 | - |
dc.identifier.uri | http://dx.doi.org/10.18419/opus-12498 | - |
dc.description.abstract | Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO2-Ta2O5 layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta2O5-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate. | en |
dc.language.iso | en | de |
dc.relation.uri | doi:10.3390/bios12010043 | de |
dc.rights | info:eu-repo/semantics/openAccess | de |
dc.rights.uri | https://creativecommons.org/licenses/by/4.0/ | de |
dc.subject.ddc | 570 | de |
dc.title | Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers | en |
dc.type | article | de |
dc.date.updated | 2022-02-05T18:33:16Z | - |
ubs.fakultaet | Energie-, Verfahrens- und Biotechnik | de |
ubs.institut | Institut für Biomaterialien und biomolekulare Systeme | de |
ubs.publikation.seiten | 16 | de |
ubs.publikation.source | Biosensors 12 (2022), No. 43 | de |
ubs.publikation.typ | Zeitschriftenartikel | de |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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biosensors-12-00043-v2.pdf | 3,83 MB | Adobe PDF | Öffnen/Anzeigen |
Diese Ressource wurde unter folgender Copyright-Bestimmung veröffentlicht: Lizenz von Creative Commons