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http://dx.doi.org/10.18419/opus-12498
Autor(en): | Welden, Melanie Poghossian, Arshak Vahidpour, Farnoosh Wendlandt, Tim Keusgen, Michael Wege, Christina Schöning, Michael J. |
Titel: | Towards multi-analyte detection with field-effect capacitors modified with tobacco mosaic virus bioparticles as enzyme nanocarriers |
Erscheinungsdatum: | 2022 |
Dokumentart: | Zeitschriftenartikel |
Seiten: | 16 |
Erschienen in: | Biosensors 12 (2022), No. 43 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-ds-125174 http://elib.uni-stuttgart.de/handle/11682/12517 http://dx.doi.org/10.18419/opus-12498 |
ISSN: | 2079-6374 |
Zusammenfassung: | Utilizing an appropriate enzyme immobilization strategy is crucial for designing enzyme-based biosensors. Plant virus-like particles represent ideal nanoscaffolds for an extremely dense and precise immobilization of enzymes, due to their regular shape, high surface-to-volume ratio and high density of surface binding sites. In the present work, tobacco mosaic virus (TMV) particles were applied for the co-immobilization of penicillinase and urease onto the gate surface of a field-effect electrolyte-insulator-semiconductor capacitor (EISCAP) with a p-Si-SiO2-Ta2O5 layer structure for the sequential detection of penicillin and urea. The TMV-assisted bi-enzyme EISCAP biosensor exhibited a high urea and penicillin sensitivity of 54 and 85 mV/dec, respectively, in the concentration range of 0.1–3 mM. For comparison, the characteristics of single-enzyme EISCAP biosensors modified with TMV particles immobilized with either penicillinase or urease were also investigated. The surface morphology of the TMV-modified Ta2O5-gate was analyzed by scanning electron microscopy. Additionally, the bi-enzyme EISCAP was applied to mimic an XOR (Exclusive OR) enzyme logic gate. |
Enthalten in den Sammlungen: | 04 Fakultät Energie-, Verfahrens- und Biotechnik |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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biosensors-12-00043-v2.pdf | 3,83 MB | Adobe PDF | Öffnen/Anzeigen |
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