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Authors: Klünder, Irene
Hülser, Dieter F.
Title: β-galactosidase production in two- and threedimensionally cultivated cell cultures
Issue Date: 1991 Konferenzbeitrag Reuß, Matthias (Hrsg.): Biochemical engineering - Stuttgart : proceedings of the 2. International Symposium on Biochemical Engineering which was held at the Univ. of Stuttgart from March 5 to 7, 1990. Stuttgart : G. Fischer, 1991. - ISBN 3-437-30662-6, S. 224-227
Abstract: In these studies, we demonstrate that the threedimensional arrangement of the cells in spheroids influences the β-galactosidase activity of transfected ltk--cells. In contrast to cells grown in monolayer culture cells growing as spheroids lost their β-galactosidase activity within few days. The reduction of β-galactosidase activity in cells grown as spheroids was not caused by a lost of the lacZ-gene as can be seen from the recovery of the β-galactosidase production when cells were retransferred in monolayer culture. As we showed in cells cultivated as monolayer, β-galactosidase activity seems to be not impaired by gradients of nutrient supply or cell cycle. These measurements are confirmed by histological sections, where the highest β-galactosidase activity was found in the viable center of the spheroids.
Appears in Collections:04 Fakultät Energie-, Verfahrens- und Biotechnik

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