Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-7765
|Authors:||Hülser, Dieter F.|
Webb, Dennis J.
|Title:||The use of the tip potential of glass microelectrodes in the determination of low cell membrane potentials|
|metadata.ubs.publikation.source:||Biophysik 10 (1973), Nr. 4, S. 273-280. URL http://dx.doi.org./10.1007/BF01194222|
|Abstract:||The tip potential of Ling-Gerard glass microelectrodes changes upon insertion into cells and thus impedes the determination of the actual membrane potential. The lower the membrane potential of a cell, the larger will be the error due to this tip potential. However, as is demonstrated, a relationship exists between the tip potential of the electrode and the measured potential difference, which allows the determination of the membrane potential of a particular cell type by linear regression. This method showed that resting lymphocytes had no membrane potential, whereas for the slime mould Dictyostelitium discoideum a membrane potential of about -9 mV could be calculated.|
|Appears in Collections:||15 Fakultätsübergreifend / Sonstige Einrichtung|
Items in OPUS are protected by copyright, with all rights reserved, unless otherwise indicated.