Please use this identifier to cite or link to this item: http://dx.doi.org/10.18419/opus-7765
Authors: Hülser, Dieter F.
Webb, Dennis J.
Title: The use of the tip potential of glass microelectrodes in the determination of low cell membrane potentials
Issue Date: 1973
metadata.ubs.publikation.typ: Zeitschriftenartikel
metadata.ubs.publikation.source: Biophysik 10 (1973), Nr. 4, S. 273-280. URL http://dx.doi.org./10.1007/BF01194222
URI: http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-67910
http://elib.uni-stuttgart.de/handle/11682/7782
http://dx.doi.org/10.18419/opus-7765
Abstract: The tip potential of Ling-Gerard glass microelectrodes changes upon insertion into cells and thus impedes the determination of the actual membrane potential. The lower the membrane potential of a cell, the larger will be the error due to this tip potential. However, as is demonstrated, a relationship exists between the tip potential of the electrode and the measured potential difference, which allows the determination of the membrane potential of a particular cell type by linear regression. This method showed that resting lymphocytes had no membrane potential, whereas for the slime mould Dictyostelitium discoideum a membrane potential of about -9 mV could be calculated.
Appears in Collections:15 Fakultätsübergreifend / Sonstige Einrichtung

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