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http://dx.doi.org/10.18419/opus-813
Autor(en): | Maurer, Steffen Christian Schulze, Holger Schmid, Rolf D. Urlacher, Vlada B. |
Titel: | Immobilisation of P450 BM-3 and an NADP+ cofactor recycling system : towards a technical application of heme-containing monooxygenases in fine chemical synthesis |
Erscheinungsdatum: | 2003 |
Dokumentart: | Preprint |
Erschienen in: | Advanced synthesis & catalysis 345 (2003), S. 802-810. URL http://dx.doi.org./10.1002/adsc.200303021 |
URI: | http://nbn-resolving.de/urn:nbn:de:bsz:93-opus-26527 http://elib.uni-stuttgart.de/handle/11682/830 http://dx.doi.org/10.18419/opus-813 |
Zusammenfassung: | Cytochrome P450 monooxygenases are potentially a very useful class of hydroxylation catalysts; they are able to introduce oxygen at activated and non-activated carbon-hydrogen bonds and thus lead to regio- and/or stereochemically pure compounds. However, this potential is lowered by their intrinsic low activity and inherent instability. P450-catalysed biotransformations require a constant supply of NAD(P)H, making the process an expensive one. To render these catalysts more suitable for industrial biocatalysis, the immobilisation of P450 BM-3 (CYP 102A1) from Bacillus megaterium in a sol-gel matrix was combined with a cofactor recycling system based on NADPƒy-dependent formate dehydrogenase (EC 1.2.1.2) from Pseudomonas sp. 101 and tested for practical applicability. This approach was used for the conversion of £]-ionone, octane and naphthalene to the respective hydroxy-compounds with DMSO as cosolvent using sol-gel immobilised P450 BM-3 mutants. |
Enthalten in den Sammlungen: | 03 Fakultät Chemie |
Dateien zu dieser Ressource:
Datei | Beschreibung | Größe | Format | |
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Maurer_2003.pdf | 578,4 kB | Adobe PDF | Öffnen/Anzeigen |
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