04 Fakultät Energie-, Verfahrens- und Biotechnik
Permanent URI for this collectionhttps://elib.uni-stuttgart.de/handle/11682/5
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Item Open Access Biophysical investigations of the in vitro effects of shock waves and ultrasound(1993) Brümmer, Franz; Suhr, Dierk; Irmer, Ulrich; Bachleitner, Christoph; Hülser, Dieter F.To investigate the interactions of ultrasonic waves with biological tissues, we developed and standardized several in vitro models. Using these systems - artificial stones, human erythrocytes, L1210 mouse leukemia cells, multicellular spheroids, cavitation assay - we are able to elucidate the mechanisms of interaction as well as the cause of clinically observed side effects.Item Open Access Cytokinins in different cell compartments after treatment with [8-14C]-kinetin : [abstract](1985) Graf, Josef A.; Dietz, B.; Kull, Ulrich-Item Open Access Prosomes of higher plants share biochemical properties : [abstract](1988) Schliephacke, Martin; Kremp, Anette; Schmid, Hans-Peter; Kull, Ulrich-Item Open Access Extraordinary biological membrane structures resulting from different local membrane curvatures(1992) Meyer, Helmut W.; Hülser, Dieter F.The bilayer arrangement of amphiphilic molecules is not only the basic structure of rather flat biological membranes, but also of regularly curved bilayers in most cubic phase structures. The basis of these cubic phase structures are infinite periodical minimal surfaces (IPMS). Extraordinary biological membrane structures resembling such IPMS were found as periodically curved bilayers in areas of the plasma membrane in a Streptomyces strain and in liposomes prepared from its extracted lipids. This structure consists of a transition of convex to concave curvatures and vice versa. A structure with curvatures in one direction only was observed in vacuolar membranes of yeast cells with a genetic defect. Our electron microscopical analysis of freeze fractured membranes of these cells revealed not only fully invaginated but also flat particle-free areas which were mainly circularly shaped, some elongated areas, however, were also present. In addition, sometimes periodical arrangements were detected which obviously are not related to IPMS structures. Both structures, however, indicate a high proportion of wedge-shaped lipid molecules in the bilayer.Item Open Access Konstruktion, Leichtbau und Ökonomie bei Pflanzen(1989) Kull, Ulrich; Herbig, AstridDie Festigkeitseigenschaften von Achsen aus Pflanzenarten unterschiedlicher Stellung werden mit Hilfe der BIO-Methode (nach F. Otto) beschrieben und zu Energiegrößen in Beziehung gesetzt. Damit lassen sich Aufwandswerte K (dirnensionslos) berechnen, die dann auf die Lebensdauer der Achsen bezogen werden. Konstruktiv gute Wirtschaftlichkeit zeigen Achsen vom Bautypus der Gräser, Blattstiele von Farnen und Achsen vom Hopfen (Liane). Der K-Wert ausdauernder Achsen liegt höher, K/Lebensdauer ist aber ähnlich wie bei einjährigen. Kurzlebige Blüten- und Fruchtstiele haben einen hohen Aufwand je Zeit. Die Größe K/Lebensdauer wird in Verbindung mit der Selektion gebracht. Vergleiche mit menschlichen Konstruktionen schließen sich an.Item Open Access β-galactosidase production in two- and threedimensionally cultivated cell cultures(1991) Klünder, Irene; Hülser, Dieter F.In these studies, we demonstrate that the threedimensional arrangement of the cells in spheroids influences the β-galactosidase activity of transfected ltk--cells. In contrast to cells grown in monolayer culture cells growing as spheroids lost their β-galactosidase activity within few days. The reduction of β-galactosidase activity in cells grown as spheroids was not caused by a lost of the lacZ-gene as can be seen from the recovery of the β-galactosidase production when cells were retransferred in monolayer culture. As we showed in cells cultivated as monolayer, β-galactosidase activity seems to be not impaired by gradients of nutrient supply or cell cycle. These measurements are confirmed by histological sections, where the highest β-galactosidase activity was found in the viable center of the spheroids.Item Open Access Transduction of chemical signals in dictyostelium cells(1984) Gerisch, Günther; Tsiomenko, Arnold; Stadler, Joachim; Claviez, Michael; Hülser, Dieter F.; Rossier, ClaudeThree different functions of cyclic AMP in D discoideum are known: (1) cAMP acts as a chemoattractant during cell aggregation, (2) it controls cell development, particularly the acquisition of aggregation competence, and (3) it is involved in terminal cell differentiation. In this report we will concentrate on the functions 1 and 2 of cAMP. Chemotaxis requires the recognition of concentration gradients in the environment by attractant binding to cell surface receptors, the processing of signals from the receptors to the contractile system of the cells, extension of pseudopods at one part, and contraction at other parts of the cells in accord with the external gradient. One pathway of signal processing from the receptors to the contractile system involves the regulation of a myosin kinase. The control of development up to aggregation competence is largely dependent on the temporal pattern of cAMP application: Only repetitive pulses enhance development. This effect has been studied using the expression of a membrane glycoprotein called contact site A as a differentiation marker.Item Open Access Item Open Access Significant role of taxus leaf mucilage in the water-balance : abstract only(1983) Distelbarth, Heidrun; Kull, Ulrich-Item Open Access
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